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KMID : 1134820080370091126
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Journal of the Korean Society of Food Science and Nutrition
2008 Volume.37 No. 9 p.1126 ~ p.1135
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Anti-oxidative and Anti-inflammatory Effects of Genistein in BALB/c Mice Injected with LPS
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Cho Hye-Yeon
Noh Kyung-Hee
Cho Mi-Kyung
Lee Mi-Ok
Kim So-Hee
Song Young-Sun
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Abstract
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This study was carried out to investigate the anti-oxidative and anti-inflammatory actions of genistein in BALB/c mice injected with lopopolysaccharide (LPS), called endotoxin. Mice (10 weeks of age) weighing approximately 20 g were divided into 4 groups. Endotoxin shock was induced by intraperitoneal injection of LPS (100 ㎎/㎏ BW). LPS and genistein＋LPS groups were injected with LPS 30 min after phosphate buffered saline (PBS) solution and genistein (200 ㎎/㎏ BW) injections, respectively. Genistein group was injected with genistein, followed by PBS, while PBS group received two injections of PBS. Superoxide anion generation of peritoneal macrophage cells was significantly (p＜0.05) lower in the genistein＋LPS group than in the LPS injection group at 8 h after intraperitoneal injection, while SOD activity was significantly higher in genistien＋ LPS group than LPS group. Tumor necrosis factor-α levels of plasma were significant lower (p＜0.05) in the genistein＋LPS injection group than LPS group at 8 h after intraperitoneal injection. Plasma TBARS was lower in genistein＋LPS group than LPS group, while hepatic TBARS were not different among groups. Hepatic glutathione concentrations and antioxidant enzyme activities were significantly higher in the genistein＋LPS group than in the LPS group at 1 h and 8 h after intraperitoneal injection. Nuclear factor-kappa B (NF-κB) transactivation was significantly (p＜0.05) inhibited in LPS group. These results demonstrate genistein may ameliorate inflammatory diseases through inhibition of NF-κB transactivation and oxidative stress, which may be mediated partially by anti-oxidative effect of genistein.
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KEYWORD
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anti-oxidative and anti-inflammatory effects, LPS, genistein, NF-κB, BALB/C mice
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